14-3-3 eta Antibody (NB100-65809)
14-3-3 eta Antibody
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anti-14 3 3 protein eta antibody, anti-YWHAH antibody, anti-YWHA1 antibody, anti-YWHA 1 antibody, anti-Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody, anti-Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody, anti-Protein AS1 antibody, anti-HGNC:12853 antibody, |
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14-3-3 eta Antibody Summary: | | Gene: | YWHAH |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | Specificity: | Recognizes 14-3-3 eta in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 eta is expressed principally in the brain although it is expressed at low levels in other tissues. The protein binds alpha-synuclein, forming part of lewy bodies in parkinsons disease and has been linked to early-onset schizophrenia. The eta isoform binds both gremlin 1, which is overexpressed in human cancers, and the DAL-1/Protein 4.1B tumour suppressor. Furthermore the glucocortcoid receptor is positively regulated by 14-3-3- eta.
NB100-65809 may not react with recombinant proteins that are not N-acetylated. |
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| Publications: | Antibody has at least 2 Background References. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional 14-3-3 eta Products 14-3-3 eta Antibody Details: | Isotype: | IgG |
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| Immunogen: | Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta.Peptide sequence: Ac.GDREQLLQRARC |
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Reacts with Mouse, Human, Bovine, Chicken, Rabbit, Rat and Sheep. |
| Uses: | Immunohistochemistry-Paraffin, Western Blot In WB detects a band of approximately 30kDa in HEK293 cell lysates. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. |
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| Dilutions: | Immunohistochemistry-Paraffin
Western Blot |
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| Unit Size: | 0.1 ml |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.
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Images (1) Reviews (0) Related Products (9) Exploring 14-3-3 eta Working with 14-3-3 eta
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Jump to: Publications, Images, Ask a Question
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Working with 14-3-3 eta
14-3-3 eta Antibody Images (1) WB: 14-3-3 ETA Antibody [NB100-65809] - WB of 14-3-3 isoforms in HEK293 cell extracts using AbD Serotec antibodies. Lane 1 = anti-gamma 14-3-3, anti-zeta 14-3-3(2), anti-C-epsilon-14-3-3(3), anti-N-epsilon-14-3-3(4), anti-eta 14-3-3(5). The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3. |
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Publications (2)14-3-3 eta Antibody Background References:- Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162-172.
- Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein. FEBS 331: 296-303.
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Other Positive Controls |
Lysates (1)Peptides and ProteinsPrimary Antibodies (5)RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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