14-3-3 eta Antibody (NB100-65809)

14-3-3 eta Antibody (NB100-65809) (0.1 ml)

Additional Products Available View our 14-3-3 eta products. Call 303-730-1950 for recommended products	Ordering Information:
	Catalog Number	NB100-65809
	Size(s)	0.1 ml	Price:
	Availability
Why Choose Novus?	Bulk Quote
	View All 14-3-3 eta Primary Antibodies

anti-Protein AS1 antibody, anti-14-3-3 protein eta antibody, anti-YWHA114-3-3 eta antibody, anti-tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide antibody

14-3-3 eta Antibody Summary:

Species:	Hu, Mu, Rt, Bv, Ch, Rb, Sh	Gene:	YWHAH
Applications:	WB, IHC-P	Purity:	Whole antisera
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	Recognizes 14-3-3 eta in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 eta is expressed principally in the brain although it is expressed at low levels in other tissues. The protein binds alpha-synuclein, forming part of lewy bodies in parkinsons disease and has been linked to early-onset schizophrenia. The eta isoform binds both gremlin 1, which is overexpressed in human cancers, and the DAL-1/Protein 4.1B tumour suppressor. Furthermore the glucocortcoid receptor is positively regulated by 14-3-3- eta. NB100-65809 may not react with recombinant proteins that are not N-acetylated.
Publications:	NB100-65809 has at least 2 Background References

View Additional 14-3-3 eta Products

Lysates	Peptides and Proteins
Primary Antibodies	RNAi
» View All 14-3-3 eta Related Products

14-3-3 eta Antibody Details:

Isotype:	IgG
Immunogen:	Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta.Peptide sequence: Ac.GDREQLLQRARC

Species Reactivity:

Reacts with Mouse, Human, Bovine, Chicken, Rabbit, Rat and Sheep.

Applications:

Uses:	Immunohistochemistry-Paraffin, Western Blot In WB detects a band of approximately 30kDa in HEK293 cell lysates. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Dilutions:	Immunohistochemistry-Paraffin Western Blot
Unit Size:	0.1 ml
Concentration:	This product is unpurified. Concentration is not relevant.
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Preservative:	0.09% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: YWHAH

Entrez:	7533 (Human)
Uniprot:	Q04917 (Human)

Related Genes:

Background:

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Images (1)


View All Images...

Be the first to review this product for a reward!

Review this product now.

14-3-3 eta Antibody Images (1)

	WB: 14-3-3 ETA Antibody [NB100-65809] - WB of 14-3-3 isoforms in HEK293 cell extracts using AbD Serotec antibodies. Lane 1 = anti-gamma 14-3-3, anti-zeta 14-3-3(2), anti-C-epsilon-14-3-3(3), anti-N-epsilon-14-3-3(4), anti-eta 14-3-3(5). The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3.

Reviews (0) Have you used this product? Review this product to earn Novus Rewards.

Publications (2)

14-3-3 eta Antibody Background References:

Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162-172.
Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein. FEBS 331: 296-303.