14-3-3 beta/alpha Antibody Summary
| Immunogen |
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 beta. Peptide sequence: Ac.TMDKSELVC |
| Specificity |
Recognizes the acetylated N-terminal of 14-3-3 beta, and may not react with recombinant proteins that are not N-acetylated. |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
YWHAB |
| Purity |
Unpurified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- ELISA
- Immunohistochemistry
- Immunohistochemistry-Paraffin ELISAWestern Blot
- Western Blot
|
| Application Notes |
Immunohistochemistry-Paraffin, ELISA, Western Blot In WB detects a band of approximately 30kDa in HEK293 cell lysates. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. |
Reactivity Notes
Reacts with Mouse, Rabbit, Human, Rat, Bovine and Sheep.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
Whole antisera |
| Preservative |
0.09% Sodium Azide |
| Purity |
Unpurified |
Alternate Names for 14-3-3 beta/alpha Antibody
Background
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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