Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
A synthetic peptide corresponding to residues near N-terminus of human 14-3-3 beta/alpha was used as immunogen.
Species Reactivity:
Reacts in Human, Mouse, Rat. Predicted to cross-react with sheep and bovine, based on sequence homology.
Applications:
Uses:
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Flow Cytometry. In Western blot this antibody detects a band at approximately 30kDa.
Dilutions:
Western Blot 1:1000, Immunohistochemistry-Paraffin 1:250, Flow Cytometry 1:50
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA.
Preservative:
0.01% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3).
Flow Cytometry: 14-3-3 B/a Antibody (E58) [NB110-55435] - Flow cytometric analysis of permeabilized HeLa cells using anti-14-3-3_/_ (red) (NB110-55435) or a rabbit IgG (negative) (green).
Immunohistochemistry: 14-3-3 B/a Antibody (E58) [NB110-55435] - Immunohistochemical analysis of human skin using anti-14-3-3
Western Blot: 14-3-3 B/a Antibody (E58) [NB110-55435] - Western blot analysis of HeLa cell lysate probed with anti-14-3-3