14-3-3 Antibody (NB100-665)
14-3-3 Antibody
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Distributor Data...
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anti-Protein kinase C inhibitor protein 1 antibody; anti-KCIP-1 antibody; anti-14-3-3 protein beta/alpha antibody; anti-KCIP 1 antibody; anti-Protein 1054 antibody, |
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14-3-3 Antibody Summary: | | Gene: | YWHAQ |
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| Purity: | Affinity purified |
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| Host: | Rabbit |
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| | Specificity: | Specific for the ~29k 14-3-3 protein. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the C-terminal peptide that was used to generate the antibody. |
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| Publications: | Antibody has at least 4 Background References. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional 14-3-3 Products 14-3-3 Antibody Details: | Immunogen: | Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH |
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| Localization: | Cytoplasmic |
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Rat. Expected to react based on homology: Bovine, Canine, Chicken, Human, Mouse, Primates, Xenopus and Zebra Fish |
| Uses: | This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~29 kDa. No other applications have been tested. *The investigator should determine the optimal working dilution for a specific application. |
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| Dilutions: | Western Blot 1:1000 |
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| TMW: | 29 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling. The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms. The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. (1-4)
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Images (1) Reviews (1) Related Products (9) Exploring 14-3-3 Working with 14-3-3
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Jump to: Publications, Images, Reviews, Ask a Question
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Working with 14-3-3
14-3-3 Antibody Images (1) Western Blot: 14-3-3 Antibody [NB100-665] - Rat hippocampal (Hipp) lysate showing immunolabeling of the ~29k 14-3-3 protein |
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Reviewed by:
Anonymous 11/19/2010
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Application:
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Western Blot
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Sample Tested:
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Mouse brain extract
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Sample Loaded:
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10µl
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Species:
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Mouse
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Sample Pretreated:
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lysis buffer and homogenization
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Blocking Buffer:
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5% Milk in PBST
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Concentration:
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5%
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Blocking Time:
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1h
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Blocking Temperature:
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20? Celsius
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Dilution Ratio:
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1:1000
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Incubation Time:
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O/N
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Incubation Temperature:
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20? Celsius
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Incubation Dilution Buffer:
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5% Milk in PBST
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Description:
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anti-rabbit IgG, HRP-label
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Manufacturer Catalog #:
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NA9340
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Dilution Ratio:
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1:5000
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Method:
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ECL
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Exposure Time:
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1 min
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Positive Control:
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-
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Negative Control:
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-
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Did you observe bands?:
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Yes
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Specific Bands:
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~29 kDa kDa
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Non-specific Bands:
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~33, 24 kDa kDa
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Publications (4)14-3-3 Antibody Background References:- Silhan J, Obsilova V, Vecer J, Herman P, Sulc M, Teisinger J, Obsil T (2004) 14-3-3 Protein C-terminal stretch occupies lig binding groove is displaced by phosphopeptide binding. J Biol Chem 279:49113-49119
- Dougherty MK, Morrison DK (2004) Unlocking the code of 14-3-3. J Cell Sci 117:1875-1884.
more... - Bridges D, Moorhead GB (2005) 14-3-3 Proteins: a number of functions for a numbered protein. Sci STKE 2005:re10.
- Berg D, Holzmann C, Riess O (2003) 14-3-3 Proteins in the nervous system. Nat Rev Neurosci 4:752-762.
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, Secondary Antibodies
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Related ProductsPositive ControlsLysates (1)Peptides and ProteinsPrimary Antibodies (7)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| 14-3-3 [p Ser58] Antibody | NB100-668 | Hu, Hu, Hu, Hu | IHC-Fr, IHC-P, WB | (1) | (5) | | | | 14-3-3 [p Ser185] Antibody | NB100-62101 | Bv, Hu, Mu, Rb, Rt | WB | (1) | | | | | 14-3-3 Antibody (EPR6380) | NBP1-95238 | Hu, Mu, Rt | WB | (1) | | | | | 14-3-3 Antibody | NBP1-74585 | Hu | ELISA, IP, WB | (1) | (1) | | | | 14-3-3 Antibody | NB100-62100 | Hu | WB | | | | | | ... and 2 more. See All » |
Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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